Device

Part:BBa_K3425201:Design

Designed by: Rubén Crespo Blanco   Group: iGEM20_UofUppsala   (2020-10-13)


pSB1C3 with CaFF-I


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2049
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2049
    Illegal NheI site found at 2077
    Illegal NheI site found at 2100
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2055
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2049
    Illegal XhoI site found at 1033
    Illegal XhoI site found at 1925
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2049
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2049
    Illegal XbaI site found at 2064
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 4082
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Same as BBa_K3425200


Source

SALEMA, Valencio, et al. Selection of single domain antibodies from immune libraries displayed on the surface of E. coli cells with two β-domains of opposite topologies. PloS one, 2013, vol. 8, no 9, p. e75126.

Sonneson, G. J., & Horn, J. R. (2009). Hapten-induced dimerization of a single-domain VHH camelid antibody. Biochemistry, 48(29), 6693-6695.

References